Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: Primary antibodies used in the present study.

Article Snippet: p-CREB S133 , Rabbit , Novus biologicals (NB110-55727) , 1:5000 (WB).

Techniques:

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: The effects of perampanel (PER) and GYKI 52466 (GYKI) on total Ca 2+ /cAMP response element-binding protein (CREB) expression and its S133 phosphorylation in chronic epilepsy rats. ( A ) Representative images for Western blot of CREB and its S133 phosphorylation level in the hippocampal tissues. ( B – D ) Quantifications of CREB (B), p-CREB S133 ( C ), and p-CREB/CREB ratio ( D ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( * ,# p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni’s multiple comparison).

Article Snippet: p-CREB S133 , Rabbit , Novus biologicals (NB110-55727) , 1:5000 (WB).

Techniques: Binding Assay, Expressing, Phospho-proteomics, Western Blot, Control, Comparison

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: The effects of 3CAI co-treatment with perampanel (PER) and GYKI 52466 (GYKI) on expression levels of GSK3β, CREB, and PICK1 and the phosphorylation levels of GSK3β and CREB in non-responders. ( A ) Representative images for Western blot of total GSK3β, CREB, and PICK1 levels and the phosphorylation levels of GSK3β and CREB. ( B – H ) Quantifications of GSK3β ( B ), p-GSK3β S9 ( C ), p-GSK3β S9/GSK3β ratio ( D ), CREB ( E ), p-CREB S133 ( F ), p-CREB S133/CREB ratio ( G ), and PICK1 ( H ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( *, # p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni’s multiple comparison).

Article Snippet: p-CREB S133 , Rabbit , Novus biologicals (NB110-55727) , 1:5000 (WB).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control, Comparison